Enzymes and Inhibitors
Worthington Biochemical Corporation DEOXYRIBONUCLEASE I RN 100UN
Molecular Biology Grade. Chromatographically purified to remove RNase and protease. Supplied as a solution at approximately 2 Kunitz units per microliter (approximately 1 mg/ml) containing 50% glycerol and 1 mM calcium chloride. 9003-98-9
New England Biolabs, Inc. PNGase F – 15000 units
PNGase F is the most effective enzymatic method for removing almost all N-linked oligosaccharides from glycoproteins. PNGase F is an amidase, which cleaves between the innermost GlcNAc and asparagine residues of high mannose, hybrid, and complex oligosaccharides.
- Leaves N-glycan core oligosaccharides intact and suitable for further analysis
- Non-recombinant with no detectable endoglycosidase F1, F2 or F3 contamination
- >= 95% purity, as determined by SDS-PAGE and intact ESI-MS
- Stored in 50% glycerol
- Optimal activity and stability for up to 24 months
- Can be used under native or denaturing conditions
New England Biolabs, Inc. α2-3,6,8 Neuraminidase - 10000 units
2-3,6,8 Neuraminidase catalyzes the hydrolysis of 2-3, 2-6, and 2-8 linked sialic acid residues from glycoproteins and oligosaccharides.
New England Biolabs, Inc. Lambda Protein Phosphatase – 20000 units
Lambda Protein Phosphatase is a Mn2+-dependent protein phosphatase with activity towards phosphorylated serine, threonine and tyrosine residues.
New England Biolabs, Inc. Factor Xa Protease – 250 µg
Factor Xa cleaves after the arginine residue in its preferred cleavage site Ile-Glu/Asp-Gly-Arg. It will sometimes cleave at other basic residues, depending on the conformation of the protein substrate. The most common secondary site, among those that have been sequenced, is Gly-Arg.
ACROBiosystems PNGase F 500U/ul
PNGase F (500U/ul) is expressed from Elizabethkingia miricola E. coli.
Worthington Biochemical Corporation ELASTASE LYOPHILIZED 1GM
Two times crystallized, (Code: ESL), supplied as a dialyzed, lyophilized powder. The enzyme should be 0.22 micron filtered after reconstitution and prior to use. Suitable for the isolation of Type II lung cells. 2594-83-7
Sino Biological SuperNuclease
SuperNuclease is a recombined Serratia marcescens’ extracellular endonuclease. The sequence is same with Benzonase (Benzonase is a trademark of Merck KGaA, Darmstadt, Germany).
GeneCopoeia BIOTIN PROTEIN LIGASE
Biotin Protein Ligase - 40 ug: 2 vials of 20 ug each (200,000 Units)
New England Biolabs, Inc. Antarctic Phosphatase – 1000 units
Antarctic Phosphatase (AnP) is a heat labile alkaline phosphatase purified from a recombinant source. AnP nonspecifically catalyzes the dephosphorylation of 5' and 3' ends of DNA and RNA phosphomonoesters. Also, AnP Hydrolyses ribo-, as well as deoxyribonucleoside triphosphates (NTPs and dNTPs). AnP is useful in many molecular biology applications such as the removal of phosphorylated ends of DNA and RNA for subsequent use in cloning or end-labeling of probes. In cloning, dephosphorylation prevents religation of linearized plasmid DNA. The enzyme acts on 5' protruding, 5' recessed, and blunt ends. AnP may also be used to degrade unincorporated dNTPs in PCR reactions to prepare templates for DNA sequencing or SNP analysis. AnP is completely and irreversibly inactivated by heating at 80C for 2 minutes, thereby making removal of AnP prior to ligation or end-labeling unnecessary.
Biocare Medical, LLC Carezyme I: Trypsin Kit - 25 ml
Trypsin is a commonly used digestive enzyme. In formalin-fixed paraffin-embedded tissues, certain antibody protocols required enzyme pretreatment for proper immunohistochemical staining. This product is provided as a two component, ready-to-use solution, and can be used at different concentrations.
Discovery Life Sciences HUMAN UGT1A4 0.5ML@5MG/ML
HumanmicrogramT1A4 SUPERSOMES 0.5mL This activity is catalyzed bymicrogramT1A4 which is expressed from humanmicrogramT1A4 cDNA using a baculovirus expression system